Journal: Frontiers in Molecular Biosciences

Article Title: Sequence- and structure-specific RNA oligonucleotide binding attenuates heterogeneous nuclear ribonucleoprotein A1 dysfunction

doi: 10.3389/fmolb.2023.1178439

Figure Lengend Snippet:

Article Snippet: Additionally, for this study we mutated amino acid R55A in OptoA1 using HiFi DNA assembly cloning (NEBuilder HiFi DNA Assembly Cloning Kit, New England BioLabs) to generate N-pmCry2PHR-A1 (R55A)-mCherry-C.

Techniques: Emulsion, Lysis, Protease Inhibitor, Protein Extraction, Modification, Membrane, Staining, Western Blot, Clone Assay, Recombinant, Software, Microscopy

Journal: Clinical Evidence

Article Title: Pancreatic cancer

doi:

Figure Lengend Snippet: Gemcitabine monotherapy versus fluorouracil monotherapy for non-resectable (locally advanced or advanced) pancreatic cancer

Article Snippet: , , Subgroup analysis , Gemcitabine monotherapy v gemcitabine plus other cytotoxic agent (multitarget antifolate, pemetrexed, or topoisomerase inhibitors irinotecan or exatecan) , Overall survival: 4 RCTs, 1404 people; HR 0.99; P = 0.80; no further data reported.

Techniques:

Journal: Clinical Evidence

Article Title: Pancreatic cancer

doi:

Figure Lengend Snippet: Gemcitabine monotherapy versus gemcitabine-based combinations for non-resectable (locally advanced or advanced) pancreatic cancer

Article Snippet: , , Subgroup analysis , Gemcitabine monotherapy v gemcitabine plus other cytotoxic agent (multitarget antifolate, pemetrexed, or topoisomerase inhibitors irinotecan or exatecan) , Overall survival: 4 RCTs, 1404 people; HR 0.99; P = 0.80; no further data reported.

Techniques:

Journal: Molecular cell

Article Title: Interdomain communication of the Chd1 chromatin remodeler across the DNA gyres of the nucleosome

doi: 10.1016/j.molcel.2016.12.011

Figure Lengend Snippet:

Article Snippet: All amino acid changes were generated using Phusion DNA polymerase (NEB).

Techniques: Recombinant, Electron Microscopy, Sequencing, Binding Assay, Construct, Software

Journal: International journal of oncology

Article Title: Generation and characterization of rabbit polyclonal antibodies against Vasohibin-2 for determination of its intracellular localization.

doi: 10.3892/ijo.2013.1919

Figure Lengend Snippet: Figure 1. Western blot and IP analyses of the three polyclonal antibodies. a, HepG2; b, HepG2-VASH2 (355 amino acid residues); c, the recombinant protein of VASH2 (311 amino acid residues). (A) A single band of the relative molecular weight 34 kDa was observed in HepG2 while two bands at 41 and 34 kDa were detected in HepG2-VASH2 (355 amino acid residues). (B) A single band at 34 kDa was detected in HepG2 and VASH2 recombinant protein with each (S1-3) antibody. (C) Only the S2 antibody performed successfully, with a clear band at 34 kDa as observed in the figure (NC, normal rabbit IgG).

Article Snippet: Full-length VASH2 cDNA (encoding for 311 amino acid residues) bought from Origene (USA) was fused with DDK or V5 tag at the c-terminal and cloned into the Lv-CMV-EGFP vector.

Techniques: Western Blot, Recombinant, Molecular Weight

Journal: International journal of oncology

Article Title: Generation and characterization of rabbit polyclonal antibodies against Vasohibin-2 for determination of its intracellular localization.

doi: 10.3892/ijo.2013.1919

Figure Lengend Snippet: Figure 3. IHC analysis of VASH2 in HepG2/HepG2-VASH2 (355 amino acid residues) xenograft tumor tissues (a and b), human liver adjacent normal tissue (c), and human liver cancer tissue (d) magnification, x200. In (a) we observed that the VASH2 protein was mainly localized in the nuclei; (b) the cytoplasm was deeply stained; (c) the VASH2 protein was mainly localized in the nuclei while only slightly in the cytoplasm; (d) both the cytoplasm and nucleus were stained.

Article Snippet: Full-length VASH2 cDNA (encoding for 311 amino acid residues) bought from Origene (USA) was fused with DDK or V5 tag at the c-terminal and cloned into the Lv-CMV-EGFP vector.

Techniques: Staining

Journal: International journal of oncology

Article Title: Generation and characterization of rabbit polyclonal antibodies against Vasohibin-2 for determination of its intracellular localization.

doi: 10.3892/ijo.2013.1919

Figure Lengend Snippet: Figure 4. Intracellular localization of the VASH2 protein. a, HepG2-nucleus; b, HepG2-cytoplasm; c, HepG2-VASH2-nucleus (355 amino acid residues); d, HepG2-VASH2-cytoplasm (355 amino acid residues); e, HepG2-VASH2-Flag (355 amino acid residues) magnification, x400; f, HepG2-VASH2-Flag (311 amino acid residues) magnification, x400; g, mouse xenograft tumor tissue (HepG2-VASH2-V5, 311 amino acid residues) magnification, x200. (A) The cytoplasmic and nuclear cellular extracts prepared and analyzed by western blotting. (B) IF analysis with the anti-DDK antibody performed in HepG2-VASH2 (transient overexpressed c-terminal DDK-tagged 311 and 355 amino acid residues VASH2). (C) IHC analysis performed in the mouse xenograft tumor tissue (HepG2-VASH2-V5, 311 amino acid residues).

Article Snippet: Full-length VASH2 cDNA (encoding for 311 amino acid residues) bought from Origene (USA) was fused with DDK or V5 tag at the c-terminal and cloned into the Lv-CMV-EGFP vector.

Techniques: Western Blot

Journal: International journal of oncology

Article Title: Generation and characterization of rabbit polyclonal antibodies against Vasohibin-2 for determination of its intracellular localization.

doi: 10.3892/ijo.2013.1919

Figure Lengend Snippet: Figure 2. Immunofluorescence staining of HepG2, magnification, x400. The first row shows distribution patterns of anti-VASH2 antibodies (S1-S3) in HepG2. The second row shows the nuclei staining with DAPI for HepG2. The last row shows merged first and second rows.

Article Snippet: Full-length VASH2 cDNA (encoding for 311 amino acid residues) bought from Origene (USA) was fused with DDK or V5 tag at the c-terminal and cloned into the Lv-CMV-EGFP vector.

Techniques: Immunofluorescence, Staining

Journal: International journal of oncology

Article Title: Generation and characterization of rabbit polyclonal antibodies against Vasohibin-2 for determination of its intracellular localization.

doi: 10.3892/ijo.2013.1919

Figure Lengend Snippet: Figure 5. Western blot analysis of the VASH2 protein intracellular localiza- tion in HepG2. a, HepG2-nucleus; b, HepG2-cytoplasm. The double dose of HepG2 cytoplasmic lysates was analyzed by Western blotting. A clear band at 34 kDa was observed in the HepG2 nucleus (a) while a slight band at 41 kDa was observed in the HepG2 cytoplasm (b).

Article Snippet: Full-length VASH2 cDNA (encoding for 311 amino acid residues) bought from Origene (USA) was fused with DDK or V5 tag at the c-terminal and cloned into the Lv-CMV-EGFP vector.

Techniques: Western Blot